大连理工大学精细化工国家重点实验室宋锋玲

bailianhua

宋锋玲，大连理工大学精细化工国家重点实验室教授，博士生导师，首批国家自然科学基金优秀青年项目获得者，教育部新世纪优秀人才计划入选者，大连市杰出青年科技人才培育计划入选者。主要从事有机荧光染料的结构性能与应用研究，并开展荧光染料及功能化荧光纳米粒子在生物医学领域的成像研究。研究成果获2007年全国百篇优秀博士学位论文提名奖； 2012年教育部自然科学一等奖；2012年辽宁省自然科学一等奖；2016年辽宁省自然科学学术成果论文类一等奖。


宋锋玲
基本信息Personal Information
教授 博士生导师 硕士生导师
性别：男
毕业院校：大连理工大学
学位：博士
在职信息：在职
所在单位：精细化工国家重点实验室
学科：应用化学 有机化学 物理化学
办公地点：大连理工大学西部校区化工实验楼E228室
联系方式：Email: songfl@dlut.edu.cn Tel: 86-411-84986307
电子邮箱：songfl@dlut.edu.cn

学习工作经历：
1995年09月至1999年07月大连理工大学应用化学专业，学士学位
1999年09月至2005年10月大连理工大学应用化学专业，博士学位
2006年01月至2006年11月美国Wayne State University化学系, 有机化学博士后
2006年12月至2009年11月美国University of Pittsburgh化学系, 有机化学博士后
2012年07月至2012年11月美国Washington University in St Louis医学院, 访问学者
2009年12月至2012年12月大连理工大学精细化工国家重点实验室副教授。
2012年12月至今 大连理工大学精细化工国家重点实验室教授
2014年06月至今 大连理工大学精细化工国家重点实验室博士生导师


代表性论文：
1. Xiong, Xiaoqing; Song, Fengling;* Wang, Jingyun; Zhang, Yukang; Xue, Yingying; Sun, Liangliang; Jiang, Na; Gao, Pan; Tian, Lu; Peng, Xiaojun. Thermally Activated Delayed Fluorescence of Fluorescein Derivative for Time-Resolved and Confocal Fluorescence Imaging. J. Am. Chem. Soc. 2014, 136, 9590−9597. (SCI当前被引44次)
2. Chen,Gengwen; Song, Fengling;* Wang, Jingyun; Yang, Zhigang; Sun,Shiguo; Fan, Jiangli; Qiang, Xinxin; Wang, Xu; Dou, Bairui; Peng, Xiaojun.* FRET spectral unmixing: a ratiometric fluorescent nanoprobe for hypochlorite. Chem.Commun., 2012, 48 (24), 2949 - 2951. (SCI当前被引75次)
3. Song, Fengling; Watanabe, Shuji; Floreancig, Paul E.; Koide, Kazunori.* Oxidation-resistant fluorogenic probe for mercury based on alkyne oxymercuration. J. Am. Chem. Soc. 2008, 130, 16460–16461. (SCI当前被引157次)
4. Song, Fengling; Garner, Amanda L; Koide, Kazunori.* A highly sensitive fluorescent sensor for palladium based on the allylic oxidative insertion mechanism. J. Am. Chem. Soc. 2007, 129, 12354–12355. (Featured in C&E News 2007, October 1 issue, pg. 30.) (SCI当前被引159次)
5. Peng, Xiaojun;* Song, Fengling; Lu, Erhu; Wang, Yanan; Zhou, Wei; Fan, Jiangli; Gao, Yunling. Heptamethine cyanine dyes with a large stokes shift and strong fluorescence: A paradigm for excited-state intramolecular charge transfer. J. Am. Chem. Soc. 2005, 127, 4170-4171. (SCI当前被引261次)




逐年论文列表：
1. Liu, Zhiwei; Song, Fengling;* Song, Bo; Jiao, Long; An, Jing; Yuan, Jingli; Peng, Xiaojun. A FRET chemosensor for hypochlorite with large Stokes Shifts and long-lifetime emissions. Sensors & Actuators: B. Chemical 2018, 262，958-965.
2. An, Jing; Wu, Yingnan; Lu, Meiheng; Han, Keli; Song, Fengling;* Peng, Xiaojun. Long-Wavelength Chromophores with Thermally Activated Delayed Fluorescence Based on Fluorescein Derivatives. Journal of Photonics for Energy, 2018, 8(3), 032103.
3. Song, Fengling;* Liang, Ri; Deng, Jundie; Liu, Zhiwei, Peng, Xiaojun. Fine-tailoring the linker of near-infrared fluorescence probes for nitroreductase imaging in hypoxic tumour cells. Chinese Chemical Letters, 2017, 28, 1997-2000.
4. Jiao, Long; Song, Fengling;* Zhang, Biyou; Ning, Houfu; Cui, Jingnan;* Peng, Xiaojun. Improving brightness and photostability of NIR fluorescent silica nanoparticles through rational fine-tuning of the covalent encapsulation methods. Journal of Materials Chemistry B, 2017, 5, 5278 - 5283
5. Wu, Yingnan; Song, Fengling;* Luo, Wenfang; Liu, Zhiwei; Song, Bo; Peng, Xiaojun. Enhanced Thermally Activated Delayed Fluorescence in New Fluorescein Derivatives By Introducing Aromatic Carbonyl Groups. ChemPhotoChem 2017, 1, 79-83.
6. Wang, Xu; Song, Fengling;* Peng, Xiaojun. A versatile fluorescent probe for imaging viscosity and hypochlorite in living cells. Dyes & Pigments. 2016, 125, 89-94
7. Xiong, Xiaoqing; Zheng, Laijiu;* Yan, Jun; Ye, Fang; Qian, Yongfang; Song, Fengling;*A turn-on and colorimetric metal-free long lifetime fluorescent probe and its application for time-resolved luminescent detection and bioimaging of cysteine. RSC Adv., 2015, 5, 53660-53664.
8. Xiong, Xiaoqing; Song, Fengling;* Wang, Jingyun; Zhang, Yukang; Xue, Yingying; Sun, Liangliang; Jiang, Na; Gao, Pan; Tian, Lu; Peng, Xiaojun. Thermally Activated Delayed Fluorescence of Fluorescein Derivative for Time-Resolved and Confocal Fluorescence Imaging. J. Am. Chem. Soc. 2014, 136, 9590−9597
9. Song, Fengling;* Ning, Houfu; She, Haiying; Wang, Jingyun; Peng, Xiaojun. A turn-on fluorescent probe for Au3+ based on rodamine derivative and its bioimaging application. Science China Chemistry. 2014, 57, 1043−1047
10. Song, Fengling;* Xue,Yingying; Wang, Xu; Wang, Jingyun; Xiong, Xiaoqing; Peng, Xiaojun. Ratiometric fluorescent probe based on novel red-emission BODIPY for determination of bovine serum albumin. Chemical Research in Chinese Universities. 2014, 30, 738−742.
11. Chen, Bo; Song, Fengling;* Sun, Shiguo; Fan, Jiangli; Peng, Xiaojun. A Highly Sensitive Fluorescent Chemosensor for Ruthenium: Oxidation Plays Triple Roles. Chem. A Eur. J., 2013, 19,10115-10118.
12. She, Haiying; Song, Fengling;* Xu, Juan; Xiong, Xiaoqing; Chen,Gengwen; Fan, Jiangli; Sun, Shiguo; Peng, Xiaojun. A New Tridentate Sulfur Receptor as A Highly Selective and Sensitive Fluorescent Sensor for Cu2+ ions. Chem. An Asian J., 2013, 8,2762-2767.
13. Wang, Junyu; Song, Fengling;* Wang, Jingyun; Peng, Xiaojun. A Near-infrared and Ratiometric Fluorescent Chemosensor for Palladium. Analyst, 2013, 138, 3667-3672.
14. Chen,Gengwen; Song, Fengling;* Xiong, Xiaoqing; Peng, Xiaojun. Fluorescent Nanosensors Based on Fluorescence Resonance Energy Transfer (FRET). Ind. Eng. Chem. Res., 2013, 52, 11228-112245..
15. Xiong, Xiaoqing; Song, Fengling;* Chen, Gengwen; Sun, Wen; Wang, Jingyun; Gao, Pan; Zhang, Yukang; Qiao,Bo; Li, Wenfang; Sun, Shiguo; Fan, Jiangli; Peng, Xiaojun. Construction of Long-Wavelength Fluorescein Analogues and Their Application as Fluorescent Probes. Chem. A Eur. J., 2013,19, 6538-6545.
16. Xiong, Xiaoqing; Song, Fengling;* Sun, Shiguo; Fan, Jiangli; Qiang, Xinxin; Wang, Xu; Dou, Bairui; Peng, Xiaojun. Red-Emissive Fluorescein Derivatives and Detection of Bovine Serum Albumin. Asian J. Org. Chem., 2013, 2, 145 – 149.
17. Chen,Gengwen; Song, Fengling;* Wang, Jingyun; Yang, Zhigang; Sun,Shiguo; Fan, Jiangli; Qiang, Xinxin; Wang, Xu; Dou, Bairui; Peng, Xiaojun. FRET spectral unmixing: a ratiometric fluorescent nanoprobe for hypochlorite. Chem.Commun., 2012, 48 (24), 2949 - 2951
18. Chen,Gengwen; Song, Fengling;* Wang, Xu; Fan, Jiangli; Sun,Shiguo; Qiang, Xinxin; Dou, Bairui; Peng, Xiaojun. Bright and Stable Cy3-Encapsulated Fluorescent Silica Nanoparticles with a Large Stokes Shift. Dyes & Pigments. 2012,93,1532-1537
19. Koide, Kazunori; Osman, Sami; Garner, Amanda L; Song, Fengling; Dixon, Tracy; Greenberger, Joel S; Epperly, Michael W. The Use of 3,5,40-Tri-O-acetylresveratrol as a Potential Prodrug for Resveratrol Protects Mice from γ-Irradiation-Induced Death. ACS Med. Chem. Lett. 2011, 2, 270–274
20. Peng, Xiaojun; Yang, Zhigang; Wang, Jingyun; Fan, Jiangli; He, Yanxia; Song, Fengling; Wang, Bingshuai; Sun, Shiguo; Qu, Junle; Qi, Jing; Yan, Meng. Fluorescence Ratiometry and Fluorescence Lifetime Imaging: Using a Single Molecular Sensor for Dual Mode Imaging of Cellular Viscosity. J. Am. Chem. Soc.. 2011, 133, 6626–6635
21. Peng, Xiaojun; Wu, Tong; Fan, Jiangli; Wang, Jingyun; Zhang, Si; Song, Fengling; Sun, Shiguo. An Effective Minor Groove Binder as a Red Fluorescent Marker for Live-Cell DNA Imaging and Quantification. Angew. Chem. Int. Ed. 2011, 50, 4180 –4183
22. Song, Fengling; Carder, Evan J.; Kohler, Clare C.; Koide, Kazunori. Studies Toward an Ideal Fluorescence Method to Measure Palladium in Functionalized Organic Molecules: Effects of Sodium Borohydride, Temperature, Phosphine Ligand, and Phosphate Ions on Kinetics. Chem.-Eur. J., 2010, 16,13500-13508.
23. Li, Honglin; Fan, Jiangli; Song, Fengling; Zhu, Hao; Du, Jianjun; Sun, Shiguo; Peng, Xiaojun. Fluorescent Probes for Pd2+ Detection by Allylidene–Hydrazone Ligands with Excellent Selectivity and Large Fluorescence Enhancement. Chem.-Eur. J., 2010, 16,12349-12356.
24. Song, Fengling; Wang, Li; Qiao, Xiaoqiang; Wang, Bingshuai; Sun, Shiguo; Fan, Jiangli; Zhang, Lihua; Peng, Xiaojun. Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein Labeling. Org. Biomol. Chem., 2010, 8, 4249–4251
25. Garner, Amanda L; Song, Fengling; Koide, Kazunori. Enhancement of a Catalysis-Based Fluorometric Detection method for palladium through rational fine-tuning of the palladium species. J. Am. Chem. Soc. 2009, 131, 5163-5171.
26. Song, Fengling; Watanabe, Shuji; Floreancig, Paul E.; Koide, Kazunori. Oxidation-resistant fluorogenic probe for mercury based on alkyne oxymercuration. J. Am. Chem. Soc. 2008, 130, 16460–16461.
27. Koide, Kazunori; Song, Fengling; de Groh, Eric D.; Garner, Amanda L.; Mitchell, Valerie D.; Davidson, Lance A.; Hukriede, Neil A. Scalable and concise synthesis of dichlorofluorescein derivatives displaying tissue permeation in live zebrafish embryos. ChemBioChem 2008, 9, 214－128.
28. Song, Fengling; Garner, Amanda L; Koide, Kazunori. A highly sensitive fluorescent sensor for palladium based on the allylic oxidative insertion mechanism. J. Am. Chem. Soc. 2007, 129, 12354–12355. (Featured in C&E News 2007, October 1 issue, pg. 30.)
29. Wang, Yanan; Guo, Xinwen; Zhang, Chen; Song, Fengling; Wang, Xiangsheng; Liu, Hai-ou; Xu, Xiaochun; Song, Chunshan; Zhang, Weiping; Liu, Xiumei; Han, Xiuwen; Bao, Xinhe. Influence of calcination temperature on the stability of fluorinated nanosized HZSM-5 in the methylation of biphenyl. Catal. Lett. 2006, 107, 209-214.
30. Peng, Xiaojun; Song, Fengling; Lu, Erhu; Wang, Yanan; Zhou, Wei; Fan, Jiangli; Gao, Yunling. Heptamethine cyanine dyes with a large stokes shift and strong fluorescence: A paradigm for excited-state intramolecular charge transfer. J. Am. Chem. Soc. 2005, 127, 4170-4171.
31. Song, Fengling; Peng, Xiaojun; Lu, Erhu; Wang, Yanan; Zhou, Wei; Fan, Jiangli. Tuning the photoinduced electron transfer in near-infrared heptamethine cyanine dyes. Tetrahedron Lett. 2005, 46, 4817-4820.
32. Lu, Erhu; Peng, Xiaojun; Song, Fengling; Fan, Jiangli. A novel fluorescent sensor for triplex DNA. Bioorg. Med. Chem. Lett. 2005, 15(2), 255-257.
33. Song, Fengling; Peng, Xiaojun; Lu, Erhu; Zhang, Rong; Chen, Xiuying; Song, Bo. Syntheses, spectral properties and photostabilities of novel water-soluble near-infrared cyanine dyes. J. of Photochem. & Photobio. A: Chem. 2004, 168, 53–57.
34. Wang, Liqiu; Peng, Xiaojun; Song, Fengling; Lu, Erhu; Cui, Jingnan; Gao, Xinqin; Lu, Rongwen. New near-infrared indocyanines and their spectral properties in SiO2 sol-gel. Dyes and Pigments. 2004, 61(2), 103-107.

cangcang

2018年自然科学基金面上项目-肿瘤乏氧检测与光动力治疗一体化的新型多功能荧光分子探针研究
批准号        21877011        学科分类        分子探针设计与构建 ( B070101 )
项目负责人        宋锋玲        负责人职称                依托单位        大连理工大学
资助金额        63.00万元        项目类别        面上项目        研究期限        2019 年 01 月 01 日 至2019 年 12 月 31 日

xinji

精细化工国家重点实验室彭孝军院士团队宋锋玲老师一直致力于有机荧光染料和荧光纳米粒子的光物理现象、及其在生物荧光成像等生物医学领域的创新应用研究。在最近的研究中，他们基于前期工作（J. Mater. Chem. B, 2017, 5, 5278-5283; ACS Appl. Mater. Interfaces, 2019, 11, 25750-25757; Chem. Commun., 2019, 55, 14522-14525），提出了基于分子工程和纳米材料改性的组合新策略，双管齐下，攻克了传统有机荧光染料在水性介质中的ACQ和荧光漂白的难题，实现了FSNPs在肿瘤细胞内长时间、高亮荧光成像和稳定的PDT过程。即通过分子工程策略对共价掺杂的近红外氨基菁染料进行分子结构优化设计、并结合纳米材料改性策略在FSNPs内部构建“纳米疏水笼”结构提供了一个相对非极性微环境，来同时攻克FSNPs发生在水性介质中的上述缺陷。研究人员通过改性的反相微乳液法制备了一系列掺杂不同结构菁染料和正辛基三乙氧基硅烷（OTES）改性的FSNPs。通过对其在水中荧光寿命、相对荧光强度和抗光漂白等光物理性能的系统分析，验证了增加染料分子中共价掺杂锚定位点和位阻基团数量、以及OTES构建的“纳米疏水笼”结构对强化FSNPs光物理性能的积极作用。即通过增加共价掺杂锚定位点和位阻基团数量使得染料分子在纳米粒子内部运动受限而减少非辐射跃迁能量衰变，同时又避免了因π−π堆积而导致的ACQ现象。于此同时，利用OTES分子内烷基链的疏水特性构建的“纳米疏水笼”结构为掺杂的染料分子提供了一个相对的非极性微环境，进而抑制外部水分子渗透而不会阻碍氧气分子的自由扩散，避免了染料通过电子转移途径产生的ROS对其光氧化而导致的光漂白现象。最终的实验结果表明：FSNPs相比于自由染料在水溶液中有12.3倍的荧光强度提升和增强的抗光漂白能力，并在肿瘤细胞荧光成像过程中经过连续激光扫描300秒后仍保留有90.1%的初始荧光强度。最后在2D和3D肿瘤细胞模型的PDT过程中也表现出极佳的PDT稳定性。因此，这种组合策略为合理调控FSNPs内部微环境及强化光物理性能、开发高性能FSNPs提供了新思路！

该研究成果以Research Article形式发表在国际学术期刊ACS Central Science上（原文链接：https://doi.org/10.1021/acscentsci.0c00071）。大连理工大学博士研究生焦龙为文章的第一作者，宋锋玲教授为文章的通讯作者，以上工作得到了国家自然科学基金和山东省生态化工协同创新中心人才基金的支持。