中国科学院化学研究所活体分析化学实验室上官棣华

dahuangfeng

上官棣华        sgdh@iccas.ac.cn        62528509

1988-1992 中国药科大学药物分析专业，获学士学位。

1992-1995 同济医科大学附属协和医院临床药学实验室工作，主要从事治疗药物监测以及中药制剂临床前研究。

1995-1998 中国协和医科大学，中国医学科学院医药生物技术研究所，获硕士学位，主要从事新抗生素筛选，天然产物分离分析研究。

1998-2001 中国科学院化学研究所，获博士学位，主要从事生物活性物质的高灵敏度检测研究。

2001-2003 中国科学院化学研究所生命分析化学实验室，助理研究员，主要从事生物活性物质的动态生化分析、生物色谱研究。

2003-2006 佛罗里达大学化学系，肿瘤研究中心，博士后，主要从事肿瘤细胞核酸适体（aptamer）的筛选和应用研究。

2007-至今 中国科学院化学研究所活体分析化学实验室，研究员。

2002年度,2013年度 中国分析测试协会科学技术奖（CAIA奖）一等奖

研究方向        

1）新型识别分子的筛选、设计与构筑

2）分子探针的构建与应用

3）亲和分离材料的制备与应用

论文著作        

2016

1.      Ying Liu#, Jin Zhou#, Linlin Wang#, Xiaoxiao Hu, Xiangjun Liu, Meirong Liu, Zehui Cao, Dihua Shangguan*, and Weihong Tan. A Cyanine Dye to Probe Mitophagy: Simultaneous Detection of Mitochondria and Autolysosomes in Live Cells, J. Am. Chem. Soc., 2016, 138 (38), 12368–12374.

2.      Nan Zhang, Tao Bing, Luyao Shen, Rusheng Song, Linlin Wang, Xiangjun Liu, Meirong Liu, Juan Li, Weihong Tan* and Dihua Shangguan* Intercellular Connections Related to Cell–Cell Crosstalk Specifically Recognized by an Aptamer. Angew. Chem. Int. Ed. 2016, 55, 3914–3918.

3.      Xin Zhang, Bing Jin, Wei Zheng, Nan Zhang, Xiangjun Liu, Tao Bing, Yongbiao Wei, Fuyi Wang, Dihua Shangguan*, Interaction of hypericin with guanine-rich DNA: Preferential binding to parallel G-Quadruplexes, Dyes and Pigments 132 (2016) 405-411.

4.      Shangrong Wu, Linlin Wang, Nan Zhang, Ying Liu, Wei Zheng, Ang Chang, Fuyi Wang, Songqin Li and Dihua Shangguan*, A Bis-methylpiperazin-styryl Phenanthroline as a Fluorescent Ligand for G-Quadruplexes, Chem. Eur. J. 2016, 22, 6037–6047.

5.      Tianjun Chang, Hongmei Gong, Pi Ding, Xiangjun Liu, Weiguo Li, Tao Bing, Zehui Cao, and Dihua Shangguan* Activity Enhancement of G-Quadruplex/Hemin DNAzyme by Flanking d(CCC), Chem. Eur. J. 2016, 22, 4015–4021.

6.      常昂，吴尚荣，柳影，张鑫，魏涌标，李松青*，刘祥军*,上官棣华。基于新铜试剂的铜离子荧光探针的设计合成及性能研究, 分析科学学报，32卷 01期，pp 58-62，2016/2.

2015

7.      Xiangjun Liu*, Linlin Wang, Nan Zhang and Dihua Shangguan*, Ratiometric fluorescent silver nanoclusters for the determination of mercury and copper ions, Anal. Methods, 2015, 7(19), 8019-8024.

8.      Nan Zhang, Tao Bing, Xiangjun Liu, Cui Qi, Luyao Shen, Linlin Wang and Dihua Shangguan*, Cytotoxicity of guanine-based degradation products contributes to the antiproliferative activity of guanine-rich oligonucleotides, Chem. Sci., 2015, 6(7), 3831-3838.

9.      Jin Zhou, Canliang Fang, Ying Liu, Yao Zhao, Nan Zhang, Xiangjun Liu, Fuyi Wang and Dihua Shangguan*, Visible-light-induced cleavage of 4-alpha-amino acid substituted naphthalimides and its application in DNA photocleavage, Organic & Biomolecular Chemistry, 2015, 13 (13) 3931-3935.

10.  Ying Liu, Linlin Wang, Jin Zhou, Shangrong Wu, Yongbiao Wei, Ang Chang, Xiangjun Liu, Dihua Shangguan*, DNA interaction and cellular localization of quinacridone derivatives, Dyes and Pigments, 2015, 121, 328–335.

11.  Yongbiao Wei, Xin Zhang, Linlin Wang, Ying Liu, Tao Bing, Xiangjun Liu and Dihua Shangguan*, Interaction of bisbenzimidazole-substituted carbazole derivatives with G-quadruplexes and living cells, RSC Adv., 2015, 5（93）, 75911-75917.

12.  Tao Bing, Dihua Shangguan* and Yinsheng Wang*, Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers, Molecular & Cellular Proteomics, 2015, 14, 2692-2700.

13.  常昂,刘祥军,吴尚荣,柳影,王林林,张楠,李松青*,上官棣华*.化学通报,2015,78(12)1101.

2014

14.  Cui Qi, Nan Zhang, Jingli Yan, Xiangjun Liu, Tao Bing, Hongcheng Mei, and Dihua Shangguan*, Activity enhancement of G-quadruplex/hemin DNAzyme by spermine, RSC Adv., 2014, 4(3), 1441-1448.

15.  Bing Jin, Xin Zhang, Wei Zheng, Xiangjun Liu, Cui Qi, Fuyi Wang, and Dihua Shangguan*, Fluorescence Light-Up Probe for Parallel G-Quadruplexes, Anal. Chem., 2014, 86(1), 943-952.

16.  Xiangjun Liu, Nan Zhang, Tao Bing, and Dihua Shangguan*, Carbon Dots Based Dual-Emission Silica Nanoparticles as a Ratiometric Nanosensor for Cu2+, Anal. Chem., 2014, 86(5), 2289-2296.

17.  Tao Bing#, Hongcheng Mei#, Nan Zhang, Cui Qi, Xiangjun Liu, an d Dihua Shangguan*, Exact tailoring of an ATP controlled streptavidin binding aptamer, RSC Adv., 2014, 4 (29), 15111-15114.

18.  Bing Jin, Xin Zhang, Wei Zheng, Xiangjun Liu, Jin Zhou, Nan Zhang, Fuyi Wang, Dihua Shangguan*, Dicyanomethylene-Functionalized Squaraine as a Highly Selective Probe for Parallel G-Quadruplexes, Anal. Chem., 2014, 86(14), 7063-7070.

19.  Jin Zhou, Ang Chang, Linlin Wang, Ying Liu, Xiangjun Liu and Dihua Shangguan*, Effects of side chains on DNA binding, cell permeability, nuclear localization and cytotoxicity of 4-aminonaphthalimides, Org. Biomol. Chem., 2014, 12 (45), 9207-9215.

20.  吴尚荣,金冰,张楠,柳影,刘祥军,李松青*,上官棣华*, 一种不对称菁染料及其与不同结构DNA的相互作用, 高等学校化学学报, 2014, 35(10), 2085-2092.

21.  Yuanyuan Wang#, Yun Luo#, Tao Bing, Zheng Chen, Minhua Lu, Nan Zhang, Dihua Shangguan*, Xin Gao*, DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer, PLoS ONE, 2014, 9(6), e100243.

22.  Wan-Ming Li, Tao Bing, Jia-Yi Wei, Zhe-Zhou Chen, Di-Hua Shangguan*, Jin Fang*, Cell-SELEX-based selection of aptamers that recognize distinct targets on metastatic colorectal cancer cells, Biomaterials, 2014, 35(25), 6998-7007.

23.  常天俊, 李刚, 赵可旭, 班琳, 卞文秀, 邴涛, 上官棣华*, 连接环、末端碱基及一价阳离子对G-四链体结构的影响(Effects of Loops, Flanking Bases and Monovalent Cations on the Structures of G-Quadruplexes), 高等学校化学学报, 2014, Vol. 35, Issue (12): 2556-2562.

2013:

24.  Jin Zhou, Huiying Liu, Bin Jing, Xiangjun Liu, Hongbing Fu, and Dihua Shangguan*, A guanidine derivative of naphthalimide with excited-state deprotonation coupled intramolecular charge transfer properties and its application, J. Mater. Chem. C, 2013, 1, 4427-4436.

25.  Tianjun Chang, Cui Qi, Jie Meng, Nan Zhang, Tao Bing, Xianda Yang, Zehui Cao, Dihua Shangguan*, General Cell-Binding Activity of Intramolecular G-Quadruplexes with Parallel Structure, Plos One, 2013, 8, e62348.

26.  Jingli Yan, Nan Zhang, Cui Qi, Xiangjun Liu, Dihua Shangguan*, One-step real time RT-PCR for detection of microRNAs, Talanta, 2013, 110, 190-195.

27.  Jin Zhou, Canliang Fang, Tianjun Chang, Xiangjun Liu, Dihua Shangguan*, A pH sensitive ratiometric fluorophore and its application for cellular pH measurement, J. Mater. Chem. B, 2013, 1(5), 661–667.

28.  Hongcheng Mei, Tao Bing, Cui Qi, Nan Zhang, Xiangjun Liu, Tianjun Chang, Jingli Yan, Dihua Shangguan*, Rational design of Hg2+ controlled streptavidin-binding aptamer, Chem. Commun., 2013, 49, 164-166.

29.  Cui Qi, Tao Bing, Hongcheng Mei, Xiaojuan Yang, Xiangjun Liu, Dihua Shangguan*, G-quadruplex DNA aptamers for zeatin recognizing, Biosens. Bioelectron., 2013, 41, 157-162.

30.  Xiangjun Liu, Nan Zhang, Jin Zhou, Tianjun Chang, Canliang Fang, and Dihua Shangguan*, A turn-on fluorescent sensor for zinc and cadmium ion based on perylene tetracarboxylic diimide, Analyst, 2013, 138, 901-906.

31.  Liangliang Huang, Xiaojuan Yang, Cui Qi, Xiaofang Niu, Chunling Zhao, Xiaohui Zhao, Dihua Shangguan*, Yunhui Yang*, A label-free electrochemical biosensor based on a DNA aptamer against codeine, Analytica Chimica Acta 787 (2013) 203– 210.

32.  程晓宏; 谢超; 史玉坤; 上官棣华, 一种快速测定铅离子的新方法, A new rapid method for lead detection 卷:32期: 7,页: 118-120, 2013, Chinese Journal of Analysis Laboratory分析试验室.

2012：

33.  Hongcheng Mei, Tao Bing, Xiaojuan Yang, Cui Qi, Tianjun Chang, Xiangjun Liu, Zehui Cao, Dihua Shangguan*, Functional-Group Specific Aptamers Indirectly Recognizing Compounds with Alkyl Amino Group, Anal. Chem., 2012, 84, 7323-7329.

34.  Tianjun Chang, Xiangjun Liu, Xiaohong Cheng, Cui Qi, Hongcheng Mei, Dihua Shangguan*, Selective isolation of G-quadruplexes by affinity chromatography, J. Chromatogr. A, 2012, 1246, 62-68.

35.  Tao Bing, Tianjun Chang, Cui Qi, Nan Zhang, Xiangjun Liu, Dihua Shangguan*, Specific interactions between adenosine and streptavidin/avidin, Bioorg. Med. Chem. Lett., 2012, 22, 7052-7055.

2011:

36.  Can-liang Fang, Jin Zhou, Xiang-jun Liu, Ze-hui Cao, and Di-hua Shangguan*, Mercury(II)-mediated formation of imide-Hg-imide complexes, Dalton Trans., 2011, 40, 899-903.

37.  Xiaojuan Yang, Tao Bing, Hongcheng Mei, Canliang Fang, Zehui Cao and Dihua Shangguan*, Characterization and application of a DNA aptamer binding to L-tryptophan, Analyst, 2011, 136, 577–585.

38.  Tao Bing, Tianjun Chang, Xiaojuan Yang, Hongcheng Mei, Xiangjun Liu and Dihua Shangguan*, G-quadruplex DNA Aptamers Generated for Systemin, Bioorg. Med. Chem., 2011, 19, 4211-4219.

39.  Xiaojuan Yang, Canliang Fang, Hongcheng Mei, Tianjun Chang, Zehui Cao, and Dihua Shangguan*, Characterization of G-Quadruplex/Hemin Peroxidase: Substrate Specificity and Inactivation Kinetics, Chem. Eur. J., 2011, 17, 14475-14484.

2010:

40.  Tao Bing, Xiangjun Liu, Xiaohong Cheng, Zehui Cao, Dihua Shangguan*, Bifunctional combined aptamer for simultaneous separation and detection of thrombin, Biosens. Bioelectron., 2010, 25, 1487-1492.

41.  Tao Bing, Xiaojuan Yang, Hongcheng Mei, Zehui Cao, Dihua Shangguan*, Conservative secondary structure motif of streptavidin-binding aptamers generated by different laboratories, Bioorg. Med. Chem., 2010, 18, 1798-1805.

42.  Xiangjun Liu, Xiaohong Cheng, Tao Bing, Canliang Fang, and Dihua Shangguan*, Visual Detection of Hg2+ with High Selectivity Using Thymine Modified Gold Nanoparticles, Anal. Sci., 2010, 26, 1169-1172.

43.  Kwame Sefah, Dihua Shangguan, Xiangling Xiong, Meghan B O'Donoghue & Weihong Tan, Development of DNA aptamers using Cell-SELEX, Nature Protocols, 2010, 5, 1169-1185.

2009:

44.  Xiangjun Liu, Cui Qi, Tao Bing, Xiaohong Cheng, and Dihua Shangguan*, Specific mercury(II) adsorption by thymine based sorbent, Talanta, 2009, 78, 253–258.

45.  Xiaohong Cheng, Tao Bing, Xiangjun Liu, and Dihua Shangguan*, A label-free fluorescence sensor for probing the interaction of oligonucleotides with target molecules, Anal. Chim. Acta, 2009, 633, 97–102.

46.  Xiangjun Liu, Cui Qi, Tao Bing, Xiaohong Cheng, and Dihua Shangguan*, Highly Selective Phthalocyanine-Thymine Conjugate Sensor for Hg2+ Based on Target Induced Aggregation, Anal. Chem., 2009, 81, 3699-3704.

47.  Xiaohong Cheng, Xiangjun Liu, Tao Bing, Zehui Cao, and Dihua Shangguan*, General Peroxidase Activity of G-Quadruplex-Hemin Complexes and Its Application in Ligand Screening, Biochemistry, 2009, 48, 7817–7823.

48.  Xiaohong Cheng, Xiangjun Liu, Tao Bing, Rui Zhao, Shaoxiang Xiong, and Dihua Shangguan*, Specific DNA G-Quadruplexes Bind to Ethanolamines, Biopolymers 2009, 91, 874-883.

49.  Xiangjun Liu, Jizhong Liu, Yanyan Huang, Rui Zhao*, Guoquan Liu, Yi Chen, Determination of methotrexate in human serum by high-performance liquid chromatography combined with pseudo template molecularly imprinted polymer, J. Chromatogr. A, 2009, 216, 7533-7538.

Before

50.  D. Shangguan , Z. Cao, L. Meng, P. Mallikaratchy, K. Sefah, H. Wang, Y. Li and W. Tan, Cell-specific Aptamer Probes for Membrane Protein Elucidation in Cancer Cells. J. Proteome Res., 2008, 7(5), 2133-2139.

51.  D. Shangguan , L. Meng, Z. C. Cao, Z. Xiao, X. Fang, Y. Li, D. Cardona, R. P. Witek, C. Liu, and W. Tan, Identification of Liver Cancer-specific Aptamers Using Whole Live Cells. Anal. Chem., 2008, 80(3), 721-728.

52.  D. Shangguan , Z. Tang, P. Mallikaratchy, Z. Xiao and W. Tan, Optimization and Modifications of Aptamers Selected from Live Cancer Cell Lines. Chembiochem, 2007, 8(6), 603-606.

53.  D. Shangguan, C. Cao, Y. Li and W. Tan, Aptamers Evolved from Cancer Cells Reveal Molecular Differences of Cancer Patient Samples. Clin Chem, 2007, 53(6), 1153-1155.

54.  X. Liu, Z Chen, R Zhao, D Shangguan , G Liu, Y Chen,. Uniform-sized Molecularly Imprinted Polymer for Metsulfuron-Methyl by One-step Swelling and Polymerization Method. Talanta, 2007, 71, 1205-1210.

55.  刘祥军、刘吉众、赵睿*、刘国诠、陈义，三甲氧苄啶分子印迹整体柱的制备及色谱性能，高等学校化学学报，2007，28，1878-1880.

56.  D. Shangguan, Y. Li, Z. Tang, Z. C. Cao, H. W. Chen, P. Mallikaratchy, K. Sefah, C. J. Yang and W. Tan,. Aptamers Evolved from Live Cells as Effective Molecular Probes for Cancer Study. Proc. Natl. Acad. Sci. USA, 2006, 103, 11838-11843.

57.  X. Liu , C. Ouyang, R. Zhao, D. Shangguan , Y. Chen, G. Liu, Monolithic Molecularly Imprinted Polymer for Sulfamethoxazole and Molecular Recognition Properties in Aqueous Mobile Phase. Anal. Chim. Acta, 2006, 571, 235-241.