湖南师范大学化学化工学院曾佑林

tianjiao

曾佑林
性  别：男
学历：博士
职称：副教授
其他：硕士生导师
Youlin Zeng
Associate Professor
Professional Background:
B.S., 1994, Hunan Normal University, Major: Chemistry.
M.S., 1998, Major: Polymer Chemistry and Physics.
Ph.D., 2001, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Major: Carbohydrte Chemistry.
Work Experience, 2004-2006, Hunan Normal University, Organic Chemistry Teaching & Science Research.
Postdoctoral Fellow, 2006-, The University of Toledo
Email: youlinzengcn@gmail.com　　　Phone:(86) 13975805056
Office: New Chemical Engineering Building Fax:(86) 0731-88872531
Skype:z-y-l-cn  QQ:814378236
Research Synopsis:
Chemistry and biology of carbohydrates. Glycobiology is the last important frontier in biochemistry. The difficulty for carbohydrate research is caused by the complexity and variety of carbohydrate structures. Carbohydrate molecules become the biomolecules carrying the largest amounts of bio-information. As information carriers, carbohydrates play essential roles in living organisms, including determination of antigenecity of molecules and phenotype of cell, and recognition in many physiological and pathological events. The simulative syntheses of the bioactive oligosaccahrides for verifying important conclusions of bio-functions of natural oligosaccharides and further chemical modification for obtaining more active non-natural oligosaccahrides are very important for study on structure-bio-function relationships.
Gene delivery system  The key technology for gene therapy is to design an effective and safe vector for targeted gene delivery. Cationic liposome based non-viral gene vectors are designed, synthesized and evaluated by in vitro transfection. The transfection properties and targeted assay of those gene vectors will be discovered by observing enhanced green fluorescent protein (EGFP) expression from pEGFP-C1 plasmid in HEK293, 293FT, GES-1, GC-1, SW-480, HT-1080, U251, 3T3-L1, MCF-7, Hct-116, HUVEC, HeLa, A549, HBL100-SV40 and NIH3T3 cells. And a structure-activity relationship based on the chemical structure and physical structure of lipids gene delivery vectors will be established by evaluating the effects of the chemical structure and physical structure such as length of hydrophobic chain, length of the linkage between hydrophilic terminal and hydrophobic terminal, hydrophilic terminal types, hydrophilic terminal density, surface zeta potential, particle size and size distribution, on the transfection properties. This structure–activity relationship will be to provide a guiding theory for constructing new cationic liposome-based non-viral targeted gene delivery vectors and their application.


基金与项目
主持项目情况：
1. 新型糖类脂质体靶向基因载体的设计、合成及转染性能研究(国家自然科学基金，80万元，21272064，2013.01-2016.12，主持人)
2. 抗流感病毒的唾液酸类似物的合成以及方法学研究(教育部“留学回国人员科研启动基金”外教司留[2010]609号-03，3.5万元，2011.01-2013.12，主持人)
3. 以糖基磺酸酯为糖苷化供体合成天麻素及其类似物(湖南省教育厅科学研究基金，1.0万元，No.2006，2007.01.-2008.01，主持人)；
4. 几种多糖的分离纯化与构效关系研究(湖南师范大学博士启动基金，2.5万元，化050603，2004.12.-2006.12.，主持人)；
5. 几种氨基酸双保护合成工艺开发(横向，5万元，化080427，2008.09~2009.9，主持人)
参与项目情况：
1. 抗肿瘤寡核苷酸类药物及其体内靶向载体的研发与应用("863计划"资源环境技术领域2007年度专题课题，2007AA02Z172，2008-2010，300万元，组长为张健教授，副组长苏胜培教授，主要参与人)
2. Development of New Carbohydrate Synthesis Methodologies (NIH, $1,194,834, R01 GM72667, PI: Xuefei Huang)
3. 微波化学法处理砷镉重金属废水机理研究(所属课题：湘江水环境重金属污染整治关键技术研究与综合示范，子课题：水口山复合重金属污染控制技术与集成，编号：2009ZX07212 -001-04，主持单位：湖南省环境保护科学研究院，主要参与人)
Publications
1.  Yan Long, Jialing Shi, Haoqiang Zeng, Youlin Zeng, Qian Cai. Enantioselective Formation of Cyano-Bearing All-Carbon Quaternary Stereocenters: Copper-Catalyzed N-Arylation via Kinetic Resolution. Synthesis, 2015, 47(18), 2844-2850.
2.   Xu Yan, Jinxi Liao, Yongzhi Lu, Jinsong Liu, Youlin Zeng, and Qian Cai, Pd-Catalyzed One-Pot Synthesis of Polysubstituted Acrylamidines from Isocyanides, Diazo Compounds, and Imines. Org. Lett., 2013, 15, 2478-2481.
3.   丙二醇胺衍生物阳离子脂质体纳米颗粒的制备方法, 中国专利, 授权号: 201110444269.5.
4.   史明明, 刘美艳, 曾佑林, 苏胜培, 成应向. 硅藻土和膨润土对重金属离子Zn2+、Pb2+及Cd2+的吸附特性. 环境化学, 2012, 31, 162-167.
5.   吴烟苗, 曾佑林, 谢祥林, 徐满才, 低档茶叶中β-胡萝卜素的提取及纯化工艺研究, 中国资源综合利用, 2010, 28, 21-24.
6.   Gilbert Wasonga, YouLin Zeng, XueFei Huang, Pre-activation based stereoselective glycosylations: Stereochemical control by additives and solvent. Sci. China Chem., 2011, 54, 66-73.
7.   氨基酸阳离子脂质体纳米颗粒的制备方法, 中国专利, 授权号: 201010554778.9
8.   Youlin Zeng, Zhen Wang, Dennis Whitfield, Xuefei Huang, Installation of Electron-Donating Protective Groups, a Strategy for Glycosylating Unreactive Thioglycosyl Acceptors using the Preactivation-Based Glycosylation Method. J.Org.Chem., 2008, 73, 7952-7962.
9.   Adeline Miermont§, Youlin Zeng§, Yuqing Jing, Xin-shan Ye, Xuefei Huang, Syntheses of LewisX and Dimeric LewisX: Construction of Branched Oligosaccharides by a Combination of Preactivation and Reactivity Based Chemoselective One-Pot Glycosylations, J. Org. Chem., 2007, 72, 8958-8961.
10.   梁源, 周庆美, 曾佑林*, 徐满才, 谢青季, 葡萄糖酶电极测定环糊精聚合物中的环糊精单元,  高分子学报, 2007, 11, 1017-1021.
11.  曾佑林*, 孔繁祚, β-甘露糖苷和 β-氨基甘露糖苷的合成, 化学进展, 2006, 18, 907-926.
12.  ZengYou-lin, Zhang, Jian-jun, Kong, Fan-zuo, Synthesis of Analogues of the Antiumor (1→6)-Branched (1→3)-Glucohexaose, Chin. J. Chem., 2004, 22, 460-466.
13.  Youlin Zeng, Fanzuo Kong, Synthesis of a hexasaccharide fragment of group E streptococci polysaccharide and the tetrasaccharide repeating unit of E. coli O7:K98:H6, Carbohydr. Res., 2004, 339, 1503-1510.
14.  Youlin Zeng, Jianjun Zhang, Jun Ning and Fanzuo Kong, Synthesis of a mannose heptasaccharide existing in baker's yeast, Saccharomyces cerevisiae X2180-1A wild-type strain, Carbohydr. Res., 2003, 338, 5-9.
15.  Youlin Zeng, Fanzuo Kong, Synthesis of β-D-glucose oligosaccharides from Phytophthora parasitica, Carbohydr. Res., 2003, 338, 2359-2366.
16.  Youlin Zeng, Fanzuo Kong, Synthesis of 3,6-Branched β-D-Glucose Oligosaccharides, J. Carbohydr. Chem., 2003, 22, 881-890.
17.  Youlin Zeng, Jianjun Zhang, Fanzuo Kong, Synthesis of a mannose heptasaccharide of the pathogenic yeast, Candida glabrata IFO 0622 strain, Carbohydr. Res., 2002, 337, 1367-1371.
18.  曾佑林, 徐满才, 黄明刚, 一种新型亲水性胺基羧酸树脂的合成及其性能研究, 离子交换与吸附, 2002, 18, 272-276.
19.  曾佑林, 徐满才, 朱爱玲, 曾盈, 凝胶型交联聚苯乙烯-异氰尿酸树脂对酚类物质的吸附机理研究, 离子交换与吸附, 2001, 17, 110-116.
20.  陈如意, 黄幼援, 曾佑林, 苏胜培, 聚丙烯/碳酸钙复合材料的组分分析, 精细化工中间体, 2006, 36, 68-70.
Honors and Awards
2nd class scholarship of Hunan Normal University (1996, 1997) (awarded to 10% top students every year)
3rd class award of RCEES (2002-2003; 2003-2004)
3rd class award of Mathematical Contest in Modeling (China, 1997)